ITS Media Supplement (100X) (2024)

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Kit Summary

Supports cell proliferation under low serum conditions.

Why Use Pre-optimized and Serum-free Supplements for Cell Culture?

The efficiency of cell proliferation and maintenance of cell health can vary from one experiment to another depending on the media components, the supplements used to drive proliferation, and the quality of the starting cell population.

Although commonly used, serum-containing medium is inherently variable due to the undefined composition and lot-to-lot variability across batches of serum. Troubleshooting media variability can be a costly and time-consuming obstacle that can be minimized through the use of high quality media. Our ITS Media Supplement is a pre-optimized and serum-free media supplement that was designed to help maintain culture media consistency across passages and experiments. The ITS Media Supplement contains optimized concentrations of human insulin, human transferrin, and selenite.

ITS Media Supplement (100x):

• Contains optimized concentrations of insulin, transferrin, and selenite
• Chemically-defined media decreases experimental variation
• High lot-to-lot consistency decreases variation
• QC-tested on multiple cell lines

Kit Contents

5 mL ITS Media Supplement (100x)

Stability and Storage

Store in the dark at < -20 ^°C in a manual defrost freezer. Do not use past the expiration date.

Precautions

This product contains human transferrin. This transferrin was tested at the donor level using an FDA licensed method and found to be non-reactive for anti-HIV-1/2 and Hepatitis B surface antigen. As no testing can offer complete assurance of freedom from infectious reagents, these reagents should be handled as if capable of transmitting infection.

Limitations

• FOR LABORATORY RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
• The safety and efficacy of this product in diagnostic or other clinical uses has not been established.
• This reagent should not be used beyond the expiration date indicated on the label.

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Scientific Data

Briefly, ITS Media Supplement is prepared using the following procedure:

• Dilute the media into the cell culture media of interest
• Store completed media at 2 – 8 °C

1. Stiffness anisotropy coordinates supracellular contractility driving long-range myotube-ECM alignment
   Authors: Skillin, NP;Kirkpatrick, BE;Herbert, KM;Nelson, BR;Hach, GK;Günay, KA;Khan, RM;DelRio, FW;White, TJ;Anseth, KS;
   bioRxiv : the preprint server for biology 2023-08-11
   
2. Comparison of Immunosuppressive and Angiogenic Properties of Human Amnion-Derived Mesenchymal Stem Cells between 2D and 3D Culture Systems
   Authors: V Miceli, M Pampalone, S Vella, AP Carreca, G Amico, PG Conaldi
   Stem Cells Int, 2019-02-18;2019(0):7486279. 2019-02-18
   

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